Neurology

 

The BBB (blood brain barrier) protects the brain but it also impedes the transport of neuropharmaceuticals into the brain. cOFM is a unique tool to investigate brain pharmacokinetics (PK) and pharmacodynamics (PD) with an intact BBB in preclinical studies. cOFM studies provide data for drug development as well as unique insights into brain metabolism and signaling.

Our cOFM Services

 

  • Monitoring of substance transport across the intact BBB
  • Monitoring of neuronal biomarkers e.g. leptin, tau-protein with no limitation regarding substance size and lipophilicity
  • Monitoring of BBB function and permeability changes
  • Time-resolved substance concentration monitoring in the brain
  • Sampling in different brain areas in parallel and direct comparison between CSF and brain ISF
  • Use in various animal models from mouse/rat to primate

About cOFM


cOFM is characterized by its capability to sample cerebral ISF (interstitial fluid) while maintaining an intact BBB.

For cOFM experiments, the BBB is allowed to heal and to re-establish for 2 weeks after cOFM probe implantation. cOFM sampling is thus always  performed with an intact BBB. During sampling, the cOFM probe is continuously perfused with a physiological fluid (perfusate). The perfusate exchanges molecules with the cerebral ISF along the exchange area at the tip of the cOFM probe, resulting in diluted and also unfiltered cerebral ISF samples, as no membrane is involved. The collected cerebral ISF samples contain all molecules present in the brain tissue without restriction due to size or inherent chemical properties including drugs, proteins and antibodies.
Further, longitudinal sampling for up to several weeks yields time-resolved, high-quality data that deliver PK and PD concentration profiles.

Supporting Services

More information

To learn more about our innovative technology and its application, please visit our dedicated OFM website.

Contact Us

 

HEALTH – Institute for
Biomedicine and Health Sciences

Neue Stiftingtalstrasse 2/Entrance A/Third Floor
8010 Graz

Phone: +43 316 876-4000
Fax: +43 316 8769-4000

croservices@joanneum.at